Real-time quantitative PCR (RT-qPCR or qPCR) is revolutionizing many aspects of molecular biology and becoming the gold standard for accurate, sensitive, and fast quantification of gene expression.

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In a recent study published in PLOS ONE, researchers developed a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) system to identify severe acute respiratory syndrome coronavirus ...

Respiratory syncytial virus (RSV) was detected easiest when using reverse transcription polymerase chain reaction (RT-PCR) tests in a pediatric population. Reverse transcription polymerase chain ...

PCR-based techniques have transformed how scientists analyze transcripts’ translational applications in cancer and ...

A microchip technology test kit may facilitate point-of-care testing in remote locations, clinics, and airports while providing similar accuracy to the tube-based real-time PCR tests, investigators ...

Scientists around the world employ quantitative real-time PCR (qPCR) to measure the amount of particular nucleic acid sequences within a sample. While this technique is rapid, sensitive, and ...

In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases.

Since the emergence of COVID-19 in late 2019, the gold standard in testing for the disease has been a nuclear-derived technique: real-time reverse transcription–polymerase chain reaction, or real-time ...