In TIRF microscopy, fluorescent molecules are in a sample in an aqueous environment that is near a solid with a high refractive index, usually a glass coverslip. At what is called the critical angle, ...
(a) Total internal reflection of two Bloch waves at the interface of the photonic crystal. (b) Guidance condition: as a Bloch wave reflects twice, it duplicates itself. (c) Conventional and ...
The DeltaVision OMX V4 enables Total Internal Reflection Fluorescence Microscopy (TIRF). This technique uses an evanescent wave to selectively excite fluorophores that are close to the coverslip. This ...
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